My research and that of my PhD students is mainly focused on genetics and evolution of microbial (particularly actinomycete) specialized (secondary) metabolism. A practical outcome of these investigations is the development of genetic approaches towards in vivo manipulations of structures of small molecules being studied and improvement of their production in native and heterologous hosts. As the specialized metabolism depends on influx of the essential metabolites (amino acids, acyl-CoA, vitamins etc), certain aspects of primary metabolism are also investigated. Most of our studies are done on Streptomyces albidoflavus (albus) J1074, perhaps the most successful actinomycete chassis available so far. We are currently interested in the genetics of tRNA posttranscriptional modifications, Streptomyces ribosome structure, and sulfur cycle in actinomycetes, which remain terra incognita on metabolic maps of Actinomycetota. Lastly, we’re trying to understand the origins of unequal usage of synonymous codons in microbial genomes, with a focus on actinomycete ones, because of their peculiar codon usage biases. Our primary approach is genetic. Once the hypotheses take shape, the other techniques are employed, which we access thanks to international collaboration with labs having expertise in a method of our interest. Full list of publications and access options can be viewed at www.ncbi.nlm.nih.gov/pubmed?term=ostash%20b; www.researchgate.net/profile/Bohdan_Ostash.

Selected publications (2020-2024)

1. Zdouc MM, Blin K, Louwen NLL, … Ostash BO, Otani H, Padva L, Paliyal S, Pan X et al. MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration. Nucleic Acids Res. 2024 Dec 9:gkae1115. doi: 10.1093/nar/gkae1115
2. Piven O, Pokrytiuk V, Kasianchuk N, Ostash B, Dekina S, Panchuk R, Boldyriev O, Bashynska V, Zaremba A, Faidiuk Y, Obolenskaya MY, Polishchuk A, Petrenko O. The resilience of Ukrainian scientists. Cell Syst. 2024 Apr 17;15(4):298-304. doi: 10.1016/j.cels.2024.03.006
3. Dolya B, Hryhorieva O, Sorochynska K, Lopatniuk M, Ostash I, Tseduliak VM, Sterndorff EB, Jørgensen TS, Gren T, Dacyuk Y, Weber T, Luzhetskyy A, Fedorenko V, Ostash B. Properties of multidrug-resistant mutants derived from heterologous expression chassis strain Streptomyces albidoflavusMicroorganisms. 2023 Apr 30;11(5):1176. doi: 10.3390/microorganisms11051176
4. Ostash B, Stanchak K, Gren T, Fedorenko V. Genetic analysis of sulfate assimilation gene cluster of Streptomyces coelicolor A3(2). Fact Exper Evol Org. Vol. 32. P. 64-68. https://doi.org/10.7124/FEEO.v32.1537
5. Ostash B, Makitrynskyy R, Yushchuk O, Fedorenko V. Structural diversity, bioactivity, and biosynthesis of phosphoglycolipid family antibiotics: recent BBA Adv. 2022 Nov 17;2:100065. doi: 10.1016/j.bbadva.2022.100065
6. Koshla O, Vogt LM, Rydkin O, Sehin Y, Ostash I, Helm M, Ostash B. Landscape of post-transcriptional tRNA modifications in Streptomyces albidoflavus J1074 as portrayed by mass spectrometry and genomic data mining. J Bacteriol. 2023 Jan 26;205(1):e0029422. doi: 10.1128/jb.00294-22
7. Tseduliak VM, Dolia B, Ostash I, Lopatniuk M, Busche T, Ochi K, Kalinowski J, Luzhetskyy A, Fedorenko V, Ostash B. Mutations within gene XNR_2147 for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albus J Appl Genet. 2023 Feb;64(1):185-195. doi: 10.1007/s13353-022-00738-4
8. Tsugita A, Uehara S, Matsui T, Yokoyama T, Ostash I, Deneka M, Yalamanchili S, Bennett CS, Tanaka Y, Ostash B. The carbohydrate tail of landomycin A is responsible for its interaction with the repressor protein LanK. FEBS J. 2022 Oct;289(19):6038-6057. doi: 10.1111/febs.16460
9. Shemediuk AL, Dolia BS, Ochi K, Fedorenko VO, Ostash BO. Properties of spontaneous rpsL mutant of Streptomyces albus KO-1297. Cytol Genet. 2022;56(1):31-36. doi: 10.3103/S009545272201011X
10. Melnyk S, Stepanyshyn A, Yushchuk O, Mandler M, Ostash I, Koshla O, Fedorenko V, Kahne D, Ostash B. Genetic approaches to improve clorobiocin production in Streptomyces roseochromogenes NRRL 3504. Appl Microbiol Biotechnol. 2022 Feb;106(4):1543-1556. doi: 10.1007/s00253-022-11814-4
11. Hrab P, Rückert C, Busche T, Ostash I, Kalinowski J, Fedorenko V, Yushchuk O, Ostash B. Complete genome sequence of Streptomyces cyanogenus S136, producer of anticancer angucycline landomycin A. 3 Biotech. 2021 Jun;11(6):282. doi: 10.1007/s13205-021-02834-4
12. Koshla O, Lopatniuk M, Borys O, Misaki Y, Kravets V, Ostash I, Shemediuk A, Ochi K, Luzhetskyy A, Fedorenko V, Ostash B. Genetically engineered rpsL merodiploidy impacts secondary metabolism and antibiotic resistance in Streptomyces. World J Microbiol Biotechnol. 2021 Mar 17;37(4):62. doi: 1007/s11274-021-03030-5
13. Yushchuk O, Ostash I, Mösker E, Vlasiuk I, Deneka M, Rückert C, Busche T, Fedorenko V, Kalinowski J, Süssmuth RD, Ostash B. Eliciting the silent lucensomycin biosynthetic pathway in Streptomyces cyanogenus S136 via manipulation of the global regulatory gene adpA. Sci Rep. 2021 Feb 10;11(1):3507. doi: 10.1038/s41598-021-82934-6
14. Silov S, Zaburannyi N, Anisimova M, Ostash B. The Use of the rare TTA codon in Streptomyces genes: significance of the codon context? Indian J Microbiol. 2021 Mar;61(1):24-30. doi: 10.1007/s12088-020-00902-6
15. Yushchuk O, Ostash B, Truman AW, Marinelli F, Fedorenko V. Teicoplanin biosynthesis: unraveling the interplay of structural, regulatory, and resistance Appl Microbiol Biotechnol. 2020 Apr;104(8):3279-3291. doi: 10.1007/s00253-020-10436-y

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