My research interests are centered on bacterial genetics, with a focus on antibiotic-producing actinobacteria. This taxon is among the most gifted chemists of the Nature, producing a vast array of bioactive compounds, that have found their use as antibacterial drugs, immunosuppressants, antitumors, antifungal to name just a few. Through genetic studies of actinobacteria we hope to improve the production of known antibiotics as well as find innovative  ways to discover novel small molecules.

My second major line of research is focused on the use of availbale genomic data to understand codon-based mechanisms of regulation of gene expression. Here we develop new computation tools (see: Zaburannyy et al. 2009) and explore the role of rare UUA codon in streptomycete genomes

Peer-reviewed articles

1: Lopatniuk M, Myronovskyi M, Nottebrock A, Busche T, Kalinowski J, Ostash B, Fedorenko V, Luzhetskyy A. Effect of “ribosome engineering” on the transcription level and production of S. albus indigenous secondary metabolites. Appl Microbiol Biotechnol. 2019 Sep;103(17):7097-7110. doi: 10.1007/s00253-019-10005-y.

2: Koshla O, Yushchuk O, Ostash I, Dacyuk Y, Myronovskyi M, Jäger G, Süssmuth RD, Luzhetskyy A, Byström A, Kirsebom LA, Ostash B. Gene miaA for post-transcriptional modification of tRNA(XXA) is important for morphological and metabolic differentiation in Streptomyces. Mol Microbiol. 2019 Jul;112(1):249-265. doi: 10.1111/mmi.14266.

3: Yushchuk O, Horbal L, Ostash B, Marinelli F, Wohlleben W, Stegmann E, Fedorenko V. Regulation of teicoplanin biosynthesis: refining the roles of tei cluster-situated regulatory genes. Appl Microbiol Biotechnol. 2019 May;103(10):4089-4102. doi: 10.1007/s00253-019-09789-w.

4: Kuzhyk Y, Lopatniuk M, Luzhetskyy A, Fedorenko V, Ostash B. Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3. Indian J Microbiol. 2019 Mar;59(1):109-111. doi: 10.1007/s12088-018-0761-x.

5: Yushchuk O, Kharel M, Ostash I, Ostash B. Landomycin biosynthesis and its regulation in Streptomyces. Appl Microbiol Biotechnol. 2019 Feb;103(4):1659-1665. doi: 10.1007/s00253-018-09601-1.

6: Sehin Y, Koshla O, Dacyuk Y, Zhao R, Ross R, Myronovskyi M, Limbach PA, Luzhetskyy A, Walker S, Fedorenko V, Ostash B. Gene ssfg_01967 (miaB) for tRNA modification influences morphogenesis and moenomycin biosynthesis in Streptomyces ghanaensis ATCC14672. Microbiology. 2019 Feb;165(2):233-245. doi: 10.1099/mic.0.000747.

7: Yushchuk O, Ostash I, Vlasiuk I, Gren T, Luzhetskyy A, Kalinowski J, Fedorenko V, Ostash B. Heterologous AdpA transcription factors enhance landomycin production in Streptomyces cyanogenus S136 under a broad range of growth conditions. Appl Microbiol Biotechnol. 2018 Oct;102(19):8419-8428. doi: 10.1007/s00253-018-9249-1.

8: Kuzhyk Y, Mutenko H, Fedorenko V, Ostash B. Analysis of Streptomyces ghanaensis ATCC14672 gene SSFG_07725 for putative γ-butyrolactone synthase. Folia Microbiol. 2018 Nov;63(6):701-706. doi: 10.1007/s12223-018-0614-3.

9: Rabyk M, Yushchuk O, Rokytskyy I, Anisimova M, Ostash B. Genomic Insights into Evolution of AdpA Family Master Regulators of Morphological Differentiation and Secondary Metabolism in Streptomyces. J Mol Evol. 2018 Apr;86(3-4):204-215. doi: 10.1007/s00239-018-9834-z.

10: Ostash I, Kolvenbach B, Corvini PF, Fedorenko V, Ostash B, Cichocka D. Gene cloning system for sulfonamide-mineralizing Microbacterium sp. strain BR1. J Appl Genet. 2018 Feb;59(1):119-121. doi: 10.1007/s13353-017-0427-0.

11: Gren T, Ostash B, Babiy V, Rokytskyy I, Fedorenko V. Analysis of Streptomyces coelicolor M145 genes SCO4164 and SCO5854 encoding putative rhodaneses. Folia Microbiol. 2018 Mar;63(2):197-201. doi: 10.1007/s12223-017-0551-6.

12: Tsypik O, Makitrynskyy R, Bera A, Song L, Wohlleben W, Fedorenko V, Ostash B.Role of GntR Family Regulatory Gene SCO1678 in Gluconate Metabolism in Streptomyces coelicolor M145. Biomed Res Int. 2017;2017:9529501. doi: 10.1155/2017/9529501.

13: Koshla O, Lopatniuk M, Rokytskyy I, Yushchuk O, Dacyuk Y, Fedorenko V, Luzhetskyy A, Ostash B. Properties of Streptomyces albus J1074 mutant deficient in tRNA(Leu)(UAA) gene bldA. Arch Microbiol. 2017 Oct;199(8):1175-1183. doi: 10.1007/s00203-017-1389-7.

14: Rokytskyy I, Koshla O, Fedorenko V, Ostash B. Decoding options and accuracy of translation of developmentally regulated UUA codon in Streptomyces: bioinformatic analysis. Springerplus. 2016 Jul 4;5(1):982. doi: 10.1186/s40064-016-2683-6.

15: Horbal L, Ostash B, Luzhetskyy A, Walker S, Kalinowski J, Fedorenko V. A gene cluster for the biosynthesis of moenomycin family antibiotics in the genome of teicoplanin producer Actinoplanes teichomyceticus. Appl Microbiol Biotechnol. 2016 Sep;100(17):7629-38. doi: 10.1007/s00253-016-7685-3.

16: Yushchuk O, Ostash B, Pham TH, Luzhetskyy A, Fedorenko V, Truman AW, Horbal L. Characterization of the Post-Assembly Line Tailoring Processes in Teicoplanin Biosynthesis. ACS Chem Biol. 2016 Aug 19;11(8): 2254-64. doi: 10.1021/acschembio.6b00018.

17: Shashkov AS, Streshinskaya GM, Tul’skaya EM, Senchenkova SN, Baryshnikova LM, Dmitrenok AS, Ostash BO, Fedorenko VO. Cell wall glycopolymers of Streptomyces albus, Streptomyces albidoflavus and Streptomyces pathocidini. Antonie Van Leeuwenhoek. 2016 Jul;109(7):923-36. doi: 10.1007/s10482-016-0691-8.

18: Tsypik O, Yushchuk O, Zaburannyi N, Flärdh K, Walker S, Fedorenko V, Ostash B. Transcriptional regulators of GntR family in Streptomyces coelicolor A3(2): analysis in silico and in vivo of YtrA subfamily. Folia Microbiol (Praha). 2016 May;61(3):209-20. doi: 10.1007/s12223-015-0426-7.

19: Medema MH, Kottmann R, Yilmaz P, [62 authors] Ostash B, et al. Minimum Information about a Biosynthetic Gene cluster. Nat Chem Biol. 2015 Sep;11(9):625-31. doi: 10.1038/nchembio.1890.

20: Ostash B, Yushchuk O, Tistechok S, Mutenko H, Horbal L, Muryn A, Dacyuk Y, Kalinowski J, Luzhetskyy A, Fedorenko V. The adpA-like regulatory gene from Actinoplanes teichomyceticus: in silico analysis and heterologous expression. World J Microbiol Biotechnol. 2015 Aug;31(8):1297-301. doi: 10.1007/s11274-015-1882-6.

Born and raised in Ukraine, at the foot of Carpathian Mountains, as per cultural norm I entered Ivan Franko National University of Lviv (IFNUL). I got my undergraduate degree in Genetics from IFNUL in 1999. I spent the next four years working towards a Ph.D. on understanding the biosynthesis of antitumor antibiotic landomycin E. This was done by making and analyzing knockouts of individual genes for landomycin biosynthesis in actinobacterium Streptomyces globisporus 1912. Since 2003 I have worked in the field of actinobacterial genetics and genomics, first as a postdoctoral fellow in the lab of Suzanne Walker (Harvard Medical School), and then as an independent researcher at IFNUL. In 2011 I took part-time teaching position at the Department of Genetics and Biotechnology of IFNUL. I teach courses in genomics and bionformatics.

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